To determine the effects of a number of non-genetic factors on milk components and estimate their indirect relationships with weaning weight (WW205), the percentages of protein, fat, lactose and solids non-fat in Charolais cows from Mexico were estimated. Lactation period (LP) and calving season (CS) had significant effects on protein and fat, whereas lactose and solids non-fat were only affected by the LP. Sire line and age of the dam had significant effects on calf birth weight, while CS affected WW205. Residual correlations between milk components and WW205 suggest a low but significant correlation with lactose. These results demonstrate the importance of several non-genetic factors on the composition of milk from Charolais cows managed under extensive cow-calf production systems and the indirect relationships between these factors and the weaning weight of a calf.